北九州市立大学国際環境工学部

環境生命工学科

望月研究室

研究業績

原著論文

  1. Orimoto A, Addison W.N., Mochizuki S, Ariyoshi W, Ono K, Kitamura C, Kiyono T, Fukuda T. Controlled cell proliferation and immortalization of human dental pulp stem cells with a doxycycline-inducible expression system. Cell Biochem. Funct., 42(4), e4064 (2024).
  2. Takeda K, Nakano S, Nishio R, Muku D, Mochizuki S, Koga A, Watanabe K. Yoshioka Y, Ariyoshi W, Yamasaki R. Medicinal herbs, especially Hibiscus sabdariffa, inhibit oral pathogenic bacteria. J. Oral Biosci., 66(1), 179-187 (2024).
  3. Matsuda M, Mochizuki S. Control of A/D type CpG-ODN aggregates to a suitable size for induction of strong immunostimulant activity. Biochem. Biophys. Rep., 36, 101573 (2023).
  4. Ogata S, Tsuji R, Moritaka A, Ito S, Mochizuki S. Modification of antigenicity of cancer cells by conjugate consisting of hyaluronic acid and foreign antigen. Biomater. Sci., 11(17), 5809-5818 (2023).
  5. Irie H, Morita K, Matsuda M, Koizumi M, Mochizuki S. Tyrosinase-related protein2 peptide with replacement of N-terminus residue by cysteine binds to H-2Kb and induces antigen-specific cytotoxic T lymphocytes after conjugation with CpG-DNA. Bioconj. Chem., 34(2), 433-442 (2023).
  6. Yoshihara E, Nabil A, Mochizuki S, Iijima M, Ebara M. Preparation of temperature-responsive antibody-nanoparticles by RAFT-mediated grafting from polymerization. Polymers, 14(21), 4584 (2022).
  7. Tsuji R, Ogata S, Mochizuki S. Interaction between CD44 and highly condensed hyaluronic acid through crosslinking with proteins. Bioorg. Chem., 121, 105666 (2022).
  8. Mochizuki S, Miyamoto N, Sakurai K. Oligonucleotide delivery to antigen presenting cells by using schizophyllan. Drug. Metab. Pharmacokinet., 42, 100434 (2022).
  9. Fujiwara N, Izumi H, Kira R, Morimoto Y, Mochizuki S, Sakurai K. Binding assay of human Dectin-1 variants to DNA/β-glucan complex for active-targeting delivery of antisense DNA. Carbohydr. Res., 500, 108219 (2021).
  10. Takahara M, Mochizuki S, Wakabayashi R, Minamihata K, Goto M, Sakurai K, Kamiya N. Extending the Half-Life of a Protein in Vivo by Enzymatic Labeling with Amphiphilic Lipopeptides. Bioconj. Chem., 32(4), 655-660 (2021).
  11. Irie H, Morita K, Koizumi M, Mochizuki S. Immune Responses and Antitumor Effect through Delivering to Antigen Presenting Cells by Optimized Conjugates Consisting of CpG-DNA and Antigenic Peptide. Bioconj. Chem., 31(11), 2585-2595 (2020).
  12. Sasaki S, Izumi H, Morimoto Y, Sakurai K, Mochizuki S. Induction of potent cell growth inhibition by Schizophyllan/K-ras antisense complex in combination with gemcitabine. Bioorg. Med. Chem., 28(18), 115668 (2020).
  13. Sumiya K, Matsunaga T, Tanaka M, Mochizuki S, Sakurai K. Oligo-DNA Stoichiometrically Binds β-1,3-Glucan with the Best Fit Length. Biomacromolecules. 21(12), 4823-4834 (2020).
  14. Sakisaka H, Takedatsu H, Mitsuyama K, Mochizuki S, Sakurai K, Sakisaka S, Hirai F. Topical Therapy with Antisense Tumor Necrosis Factor Alpha Using Novel β-Glucan-Based Drug Delivery System Ameliorates Intestinal Information. Int. J. Mol. Sci., 21(2), 683 (2020).
  15. Fujiwara N, Izumi H, Morimoto Y, Sakurai K, Mochizuki S. Complex Consisting of Antisense DNA and β-glucan Promotes Internalization into Cell through Dectin-1 and Hybridizes with Target mRNA in Cytosol. Cancer Gene Ther., 26, 32-40 (2019).
  16. Dung N, Ngoc N, Ling N, Ly D, Tung N, Nam N, Sakurai K, Mochizuki S, Kihara T, Thang N, Anh N, uong P. An agarose-curdlan nanogel that carries etanercept to target and neutralizes TNF-α produced by dectin-1-expressing immune cells. J. Elec. Materi., 48, 6570-6582 (2019).
  17. Mochizuki S, Morishita H, Sakurai K. Complex consisting of β-Glucan and antigenic peptides with cleavage site for glutathione and aminopeptidases induces potent cytotoxic T lymphocytes. Bioconj. Chem., 28(9), 2246-2253 (2017).
  18. Miyamoto N, Mochizuki S, Fujii S, Yoshida K, Sakurai K. Adjuvant activity enhanced by cross-linked CpG-oligonucleotides in β-glucan nanogel and its antitumor effect. Bioconj. Chem., 28(2), 565-573 (2017).
  19. Miyamoto N, Fujii S, Mochizuki S, Sakurai K. A two-component micelle with emergent pH responsiveness by mixing dilauroyl phosphocholine and deoxycholic acid and its delivery of proteins into the cytosol. Colloids Surf. B Biointerfaces, 154, 246-252 (2017).
  20. Maegawa Y, Mochizuki S, Miyamoto N, Sakurai K. Gene silencing using a conjugate comprising Tat peptide and antisense oligonucleotide with phosphorothioate backbones. Bioorg. Med. Chem. Lett., 26(4), 1276-1278 (2016).
  21. Izumi H, Nagao S, Mochizuki S, Fujiwara N, Sakurai K. Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system. Int. J. Oncol., 48(6), 2472-2478 (2016).
  22. Fukuda I, Mochizuki S, Sakurai K. Competition of PEG coverage density and con-A recognition in mannose/PEG bearing nanoparticles. Colloids Surf. B Biointerfaces. 146, 642-648 (2016).
  23. Shiraishi K, Kawano K, Maitani Y, Aoshi T, Ishii KJ, Sanada Y, Mochizuki S, Sakurai K, Yokoyama M. Exploring the relationship between anti-PEG IgM behaviors and PEGylated nanoparticles and its significance for accelerated blood clearance. J. Control. Release, 234, 59-67 (2016).
  24. Matsushita S, Mochizuki S, Sakurai K, Kawano T. Prevention of copper-induced cell death by GC-rich DNA oligomers in murine macrophage-like RAW264.7 cells. Commun. Integr. Biol., 8(6), e10171731-e10171734 (2016).
  25. Mochizuki S, Morishita H, Kobiyama K, Aoshi T, Ishii KJ, Sakurai K. Immunization with antigenic peptides complexed with β-glucan induces potent cytotoxic T-lymphocyte activity in combination with CpG-ODNs. J. Control. Release, 220, 495-502 (2015).
  26. Fukuda I, Mochizuki S, Sakurai K. Macrophage-Targeting Gene Delivery Using a Micelle Composed of Mannose-Modified Lipid with Triazole Ring and Dioleoyl Trimethylammonium Propane. BioMed Research International. 2015, 1-8 (2015).
  27. Aoshi T, Haseda Y, Kobiyama K, Narita H, Sato H, Nankai H, Mochizuki S, Sakurai K, Katakai Y, Yasutomi Y, Kuroda E, Coban C, Ishii KJ. Development of Nonaggregating Poly-A Tailed Immunostimulatory A/D Type CpG Oligodeoxynucleotides Applicable for Clinical Use. J Immunol Res., 2015, 1-20 (2015).
  28. Shiraishi K, Sanada Y, Mochizuki S, Kawano K, Maitani Y, Sakurai K, Yokoyama M. Determination of polymeric micelles' structural characteristics, and effect of the characteristics on pharmacokinetic behaviors. J. Control. Release., 203, 77-84 (2015).
  29. Mochizuki S, Nishina K, Fujii S, Sakurai K. The transfection efficiency of calix[4]arene-based lipids: the role of the alkyl chain length. Biomater. Sci., 3(2), 317-322 (2015).
  30. Zhang Q, Ichimaru N, Higuchi S, Cai S, Hou J, Fujino M, Nonomura N, Kobayashi M, Ando H, Uno A, Mochizuki S, Sakurai K, Adachi Y, Ohno N, Zou H, Xu J, Li X, Takahara S. Permanent acceptance of mouse cardiac allografts with CD40 siRNA to induce regulatory myeloid cells by use of a novel polysaccharide siRNA delivery system. Gene Ther., 22(3), 217-226 (2015).
  31. Maegawa Y, Mochizuki S, Miyamoto N, Sanada Y, Sakurai K. Application toward drug delivery system using β-1,3-glucan. Trends Glycosci. Glycotechnol., 27(153), 13-29 (2015).
  32. Sakashita M, Mochizuki S, Kazuo S. Hepatocyte-targeting gene delivery using a lipoplex composed of galactose-modified aromatic lipid synthesized with click chemistry. Bioorg. Med. Chem., 22(19), 5212-5219 (2014).
  33. Mochizuki S, Morishita H, Adachi Y, Yamaguchi Y, Sakurai K. Binding assay between murine Dectin-1 and β-glucan/DNA complex with quartz-crystal microbalance. Carbohydr. Res., 391, 1-8 (2014).
  34. Kobiyama K, Aoshi T, Narita H, Kuroda E, Hayashi M, Tetsutani K, Koyama S, Mochizuki S, Sakurai K, Katakai Y, Yasutomi Y, Saijo S, Iwakura Y, Akira S, Coban C, Ishii KJ. Nonagonistic Dectin-1 ligand transforms CpG into a multitask nanoparticulate TLR9 agonist. Proc. Natl. Acad. Sci. U.S.A. 111(8), 3086-3091 (2014).
  35. Fujii S, Nishina K, Yamada S, Mochizuki S, Ohta N, Takahara A, Sakurai K. Micelles consisting of choline phosphate-bearing Calix[4]arene lipids. Soft Matter., 10(41), 8216-8223 (2014).
  36. Maeda K, Mochizuki S, Sakurai K. Naphthalene-hydrophobized β-1,3-glucan nanogel for doxorubicin delivery to immunocytes. Bioorg. Med. Chem. Lett., 24(8), 1880-1883 (2014).
  37. Maegawa Y, Mochizuki S, Sanada Y, Akiyoshi K, Sakurai K. Dual-sugar nanogel comprising β-1,3-glucan and cholesterol-bearing dextran for protein-delivery targeting antigen presenting cells. Chem. Lett., 43(5), 652-654 (2014).
  38. Yamasaki T, Ariyoshi W, Okinaga T, Adachi Y, Hosokawa R, Mochizuki S, Sakurai K, Nishihara T. Dectin-1 agonist, curdlan, regulates osteoclastogenesis by inhibiting nuclear factor of activated T-cells cytoplasmic 1 through Syk kinase. J. Biol. Chem., 289(27), 19191-19203 (2014).
  39. Miyamoto N, Mochizuki S. Sakurai K. Enhanced immunostimulation with crosslinked CpG-DNA/ β- 1,3-glucan nanoparticle through hybridization. Chem. Lett., 43(7), 991-993 (2014).
  40. Noriyasu A, Konishi T, Mochizuki S, Sakurai K, Tanaike Y, Matsuyama K, Uezu K, Kawano T. Menthol-enhanced cytotoxicity of cigarette smoke demonstrated in two bioassay models. Tob. Induc. Dis., 11(1), 18 (2013).
  41. Mochizuki S, Morishita H, Sakurai K. Macrophage specific delivery of TNF-α siRNA complexed with β-1,3-glucan inhibits LPS-induced cytokine production in a murine acute hepatitis model. Bioorg. Med. Chem., 21(9), 2535-2542 (2013).
  42. Li W.J, Mochizuki S, Sakurai K. Structural transitioin of lipopolysaccharide and reduction in the biological activity by amphiphilic lipid with cationic amino acid. Bull. Chem. Soc. Jpn., 86(5), 651-656 (2013).
  43. Mochizuki S, Kanegae N, Nishina K, Kamikawa Y, Koiwai K, Masunaga H, Sakurai K. The role of the helper lipid dioleoylphosphatidylethanolamine (DOPE) for DNA transfection cooperating with a cationic lipid bearing ethylenediamine. Biochim. Biophys. Acta.1828(2), 412-418 (2013).
  44. Mochizuki S, Higuchi S, Sakurai K. ssDNA–dsRNAs are cleaved at the next to its chimera-junction point by an unknown RNase activity. Biochem. Biophys. Res. Commun., 428(4), 433-437 (2012).
  45. Mochizuki S, Kamikawa Y, Nishina K, Fujii S, Hamada E, Kusuki S, Matsuo T, Sakurai K. Relationship between DNA-transfection efficiency and chemical structures of aromatic cationic lipids. Bull. Chem. Soc. Jpn., 85(3), 354-359 (2012).
  46. Takedatsu H, Mitsuyama K, Mochizuki S, Kobayashi T, Sakurai K, Takeda H, Fujiyama Y, Koyama Y, Nishihira J, Sata T. A new therapeutic approach using a schizophyllan-based drug delivery system for inflammatory bowel disease. Mol. Ther., 20(6), 1234-1241 (2012).
  47. Sanada Y, Matsuzaki T, Mochizuki S, Okobira T, Uezu K, Sakurai K. β-1,3-glucan schizophyllan/poly(dA) triple-helical complex in dilute solution. J. Phys. Chem. B, 116(1), 87-94 (2012).
  48. Mochizuki S, Sakurai K. Dectin-1 targeting delivery of TNF-α antisense ODNs complexed with β-1,3-glucan protects mice from LPS-induced hepatitis. J. Control. Release, 151(2), 155-61 (2011).
  49. Minari J, Mochizuki S, Matsuzaki T, Adachi Y, Ohno N, Sakurai K. Enhanced cytokine secretion from primary macrophages due to dectin-1 mediated uptake of CpG DNA/β-1,3-glucan complex. Bioconj. Chem., 22(1), 9-15 (2011).
  50. Sakuragi M, Mochizuki S, Sakurai K. Increased cellular transfection with magnetized DNA/benzyl amine lipid complexes. Bull. Chem. Soc. Jpn., 84(8), 839-844 (2011).
  51. Ohya Y, Takeda S, Shibata Y, Ouchi T, Kano A, Iwata T, Mochizuki S, Taniwaki Y, Maruyama A. Evaluation of polyanion-coated biodegradable polymeric micelles as drug delivery vehicles. J. Control. Release, 155(1), 104-110 (2011).
  52. Matsuo T, Fujii S, Kamikawa Y, Nishimura T, Sanada Y, Mochizuki S, Sakurai K. Quaternary-ammonium-bearing aromatic surfactants: effect of the alkyl chain positions on their micelle and DNA-complex structures. Chem. Lett., 40(12), 1340-1342 (2011).
  53. Mochizuki S, Sakurai K. β-1,3-glucan/antisense oligonucleotide complex stabilized with phosphorothioation and its gene suppression. Bioorg. Chem., 38(6), 260-264 (2010).
  54. Mochizuki S, Sakurai K. A novel polysaccharide/polynucleotide complex and its application to bio-functional DNA delivery system. Polymer Journal, 41(5), 343-353 (2009).
  55. Mochizuki S, Kano A, Shimada N, Maruyama A. Uptake of enzymatically-digested hyaluronan by liver endothelial cells in vivo and in vitro. J. Biomater. Sci. Polym. Ed., 20(1), 83-97 (2009).
  56. Minari J, Mochizuki S, Sakurai K. Enhanced cytokine secretion owing to multiple CpG side chains of DNA duplex. Oligonucleotides, 18(4), 337-344 (2008).
  57. Minari J, Kelly A.M, Mochizuki S, Shinkai S, Sakurai K. Polysaccharide/DNA supermolecule: Triple helix consisting of one polynucleotide and two polysaccharide chains and its application to oligo-DNA delivery interface: Polynucleotide, polysaccharide, molecular recognition. Current Trends in Polymer Science, 12, 75-102 (2008).
  58. Mochizuki S, Kano A, Yamayoshi A, Maruyama A. Hyaluronan conjugation of antigenic protein to modify immunogenic information. Sci. Technol. Adv. Mater., 7(7), 685-691 (2006).
  59. 望月慎一、狩野有宏、赤池敏宏、丸山厚「バイオナノマテリアルとしての多糖コンジュゲート」高分子論文集、61(12), 601-605 (2004).

総説

  1. 櫻井和朗、佐々木彰吾、藤原伸旭、望月慎一「βグルカンの基礎研究と応用・利用の動向　第7章　β-1.3グルカンを用いた核酸送達システム」P55-65 シーエムシー出版(2018).
  2. 藤原伸旭、望月慎一、宮本寛子、櫻井和朗「ドラッグデリバリーシステム－バイオ医薬品創成に向けた組織、細胞内、核内送達技術の開発－　第8章　多糖によるアンチセンスDNAの発達」P66-74 シーエムシー出版(2018).
  3. 宮本寛子、望月慎一、櫻井和朗「生命機能に迫る分子化学 -第13章　多糖/核酸複合体の創製と核酸医薬デリバリー」P130-135 化学同人 (2018).
  4. 伊藤大貴、望月慎一、宮本寛子、櫻井和朗「多糖核酸複合体による核酸医薬デリバリー」
    核酸医薬の創製と応用展開（第III編　第5章）(2016).
  5. 宮本寛子、望月慎一、櫻井和朗 「多糖β-1,3-グルカンを用いたCpG核酸の選択的デリバリー」ナノ学会会報、12(2), 79-84 (2014).
  6. Mochizuki S, Sakurai K. “Polysaccharide/polynucleotide complexes for cell-specific DNA delivery” Functional Polymers for Nanomedicine (RSC Polymer Chemistry Series), 190-206 (2013).
  7. 望月慎一、櫻井和朗「多糖複合体を利用した細胞特異的薬物送達システムの開発」表面、50(11), 383-391 (2012).
  8. 望月慎一、櫻井和朗「核酸デリバリー」月刊バイオインダストリー、7, 24-29 (2012).
  9. 望月慎一、櫻井和朗「β-1,3-グルカン/DNA複合体の性質と核酸医薬DDSへの応用」
    先端バイオマテリアルハンドブック、p378-384 (2012).
  10. 宮本寛子、望月慎一、櫻井和朗 「多糖β-1,3-グルカンの受容体を利用したオリゴ核酸の選択的デリバリー」
    Cellulose Commun., 19(1), 12-16 (2012).
  11. 望月慎一、櫻井和朗 「糖鎖を利用した核酸医薬の選択的デリバリー」
    医学のあゆみ、238(5), 441-446 (2011).
  12. 望月慎一、櫻井和朗「β-グルカンを利用した次世代型アジュバント開発研究の新展開」
    アジュバント開発研究の新展開、第3章2.8項, 116-123 (2011).
  13. 望月慎一、櫻井和朗「多糖β-1,3グルカンの受容体を利用したオリゴ核酸の選択的デリバリー」
    Drug Delivery System, 25(6), 565-575 (2010).
  14. 望月慎一、櫻井和朗、丸山厚「生体由来の多糖を用いた薬物送達システム」
    生体機能関連化学部会ニュースレター “研究紹介”24, 3-6 (2009).